Validation Data Gallery
|Positive WB detected in||mouse colon tissue|
|Positive IHC detected in||human heart tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:300-1:600|
|Immunohistochemistry (IHC)||IHC : 1:100-1:4000|
|Sample-dependent, check data in validation data gallery|
15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22-25 kDa|
|GenBank accession number||NM_003186|
|Gene ID (NCBI)||6876|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.
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