transgelin/SM22-specific Polyclonal antibody

transgelin/SM22-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15502-1-AP


SM22, SM22 alpha, SM22a, SM22alpha, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1

Tested Applications

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:100-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name transgelin
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberNM_003186
Gene Symbol transgelin/SM22
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.


Product Specific Protocols
WB protocol for transgelin/SM22-specific antibody 15502-1-APDownload protocol
IHC protocol for transgelin/SM22-specific antibody 15502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Physiol Genomics

Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation

Authors - B. Paul Herring

Front Med (Lausanne)

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Authors - Fang He

Front Genet

Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells.

Authors - Weitie Wang