Tested Applications
| Positive WB detected in | mouse colon tissue |
| Positive IHC detected in | human heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15502-1-AP targets transgelin/SM22-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transgelin |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | NM_003186 |
| Gene Symbol | transgelin/SM22 |
| Gene ID (NCBI) | 6876 |
| RRID | AB_2199361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody is specific to transgelin 1. It does not cross-react with transgelin 2 or 3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for transgelin/SM22-specific antibody 15502-1-AP | Download protocol |
| WB protocol for transgelin/SM22-specific antibody 15502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The transgelin/SM22-specific Polyclonal antibody (Cat# 15502-1-AP) has 5 citations published in Experimental Molecular Medicine, ACS Omega, Frontiers in Medicine, Frontiers in Genetics, and Physiological Genomics. The research fields span kidney fibrosis, aortic dissection and vascular smooth muscle cell phenotypic switching, gastric cancer molecular phenotypes, microRNA regulation of ACTG2 in vascular smooth muscle cells, and inflammation-associated VSMC dedifferentiation.
| Species | Application | Title |
|---|---|---|
Exp Mol Med Unveiling the role of transgelin as a prognostic and therapeutic target in kidney fibrosis via a proteomic approach | ||
ACS Omega Estrogen Inhibits the Phenotypic Switching of Vascular Smooth Muscle Cells through ER-α/CREB in Aortic Dissection | ||
Front Med (Lausanne) Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer. | ||
Front Genet Bioinformatics Analysis Reveals MicroRNA-193a-3p Regulates ACTG2 to Control Phenotype Switch in Human Vascular Smooth Muscle Cells. | ||
Physiol Genomics Inflammation and vascular smooth muscle cell dedifferentiation following carotid artery ligation |
