TRAPPC10 Polyclonal antibody, PBS Only

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• TRAPPC10 Polyclonal antibody, PBS Only (27549-1-PBS)

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  WB analysis using 27549-1-AP (same clone as 27549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27549-1-AP (TRAPPC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

  IP result of anti-TRAPPC10 (IP:27549-1-AP, 4ug; Detection:27549-1-AP 1:600) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 27549-1-AP (same clone as 27549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 27549-1-AP (same clone as 27549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 27549-1-AP (TRAPPC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27549-1-PBS in a different storage buffer formulation.

TRAPPC10 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 27549-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

27549-1-PBS targets TRAPPC10 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26900

  Product name: Recombinant human TRAPPC10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 127-303 aa of BC101728

  Sequence: IVENDAKKKNKTNILPRTSIVDKIRNDFCNKQSDRCVVLSDPLKDSSRTQESWNAFLTKLRTLLLMSFTKNLGKFEDDMRTLREKRTEPGWSFCEYFMVQEELAFVFEMLQQFEDALVQYDELDALFSQYVVNFGAGDGANWLTFFCQPVKSWNGLILRKPIDMEKRESIQRREATL

• Full Name: trafficking protein particle complex 10
• Calculated Molecular Weight: 1259 aa, 142 kDa
• Observed Molecular Weight: 142 kDa
• GenBank Accession Number: BC101728
• Gene Symbol: TRAPPC10
• Gene ID (NCBI): 7109
• RRID: AB_3085968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48553
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAPPC10 (Trafficking protein particle complex subunit 10) is also named as EHOC-1, Protein GT334, TRAPP subunit TMEM1 and TMEM1. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. TRAPPC10 is specific component of the multisubunit TRAPP II complex (PMID: 23511850). TRAPPC10 can interacts with TRAPPC14 (PMID:31467083). TRAPPC10 interacts with TRAPPC2L in the TRAPP II complex (PMID: 30120216). TRAPPC10 variants are associated with a microcephalic neurodevelopmental disorder (PMID: 35298461). A new Longin at the N-terminus of human TRAPPC10 may dimerize with TRAPPC2 (PMID: 23511850). Therefore, a band with a high molecular weight of 150 - 250 kDa can be formed.