TRAPPC2L Polyclonal antibody

TRAPPC2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25282-1-AP

Synonyms

Trafficking protein particle complex subunit 2-like protein, HSPC176, HSPC126

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Validation Data Gallery

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  WB analysis using 25282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25282-1-AP (TRAPPC2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25282-1-AP (TRAPPC2L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 25282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25282-1-AP (TRAPPC2L Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 25282-1-AP

  Immunofluorescent analysis of HepG2 cells using 25282-1-AP (TRAPPC2L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, U-251 cells, NIH/3T3 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25282-1-AP targets TRAPPC2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPPC2L fusion protein Ag16890
• Full Name: trafficking protein particle complex 2-like
• Calculated Molecular Weight: 140 aa, 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC105809
• Gene Symbol: TRAPPC2L
• Gene ID (NCBI): 51693
• RRID: AB_2880006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.