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WB analysis of A549 using 67171-1-Ig
A549 cells were subjected to SDS PAGE followed by western blot with 67171-1-Ig (TRIB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 67171-1-Ig
U-937 cells were subjected to SDS PAGE followed by western blot with 67171-1-Ig (TRIB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 67171-1-Ig
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67171-1-Ig (TRIB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TRIB2 Monoclonal antibody

TRIB2 Monoclonal Antibody for WB, ELISA

Cat No. 67171-1-Ig

Clone No.1A10A6

Publications(2)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, CoIP, ELISA

Synonyms

1A10A6, C5FW, FLJ57420, GS3955, TRB 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in

A549 cells, U-937 cells, RAW 264.7 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

CoIP	See 2 publications below

67171-1-Ig targets TRIB2 in WB, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag7501 Product name: Recombinant human TRIB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-343 aa of BC002637 Sequence: MNIHRSTPITIARYGRSRNKTQDFEELSSIRSAEPSQSFSPNLGSPSPPETPNLSHCVSCIGKYLLLEPLEGDHVFRAVHLHSGEELVCKVFDISCYQESLAPCFCLSAHSNINQITEIILGETKAYVFFERSYGDMHSFVRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSDKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPKAKCLIRSILRREPSERLTSQEILDHPWFSTDFSVSNSAYGAKEVSDQLVPDVNMEENLDPFFN Predict reactive species
Full Name	tribbles homolog 2 (Drosophila)
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	35-40 kDa
GenBank Accession Number	BC002637
Gene Symbol	TRIB2
Gene ID (NCBI)	28951
RRID	AB_2882467
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q92519
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for TRIB2 antibody 67171-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	CoIP	Free Radic Biol Med TRIB2 promotes pulmonary artery smooth muscle cell proliferation through SERCA2 ubiquitination in pulmonary hypertension. Authors - Wenkang Zhang View Article
human	CoIP	Structure Nanobodies identify an activated state of the TRIB2 pseudokinase Authors - Sam A Jamieson View Article