TRIB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15359-1-AP

Synonyms

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

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Validation Data Gallery

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  WB analysis of HeLa using 15359-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15359-1-AP

  WB result of TRIB2 antibody (15359-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIB2 transfected HeLa cells.

• 

  WB analysis of Jurkat using 15359-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 15359-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15359-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15359-1-AP (TRIB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15359-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15359-1-AP (TRIB2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse thymus tissue, K-562 cells
• Positive IHC detected in: human placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

15359-1-AP targets TRIB2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIB2 fusion protein Ag7605
• Full Name: tribbles homolog 2 (Drosophila)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002637
• Gene Symbol: TRIB2
• Gene ID (NCBI): 28951
• RRID: AB_2209083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

Publications

Species	Application	Title
mouse,human	WB,IF	iScience; FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons; Authors - Wan Yun Ho
mouse	WB,IF	Exp Mol Med; Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.; Authors - Eun Kyoung Do ; KD Validated