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  • KD/KO Validated

TRIB3 Polyclonal antibody

TRIB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13300-1-AP

Synonyms

SKIP3, SINK, p65-interacting inhibitor of NF-kappa-B, NIPK, Neuronal cell death-inducible putative kinase



Tested Applications

Positive WB detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13300-1-AP targets TRIB3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIB3 fusion protein Ag4085
Full Name tribbles homolog 3 (Drosophila)
Calculated Molecular Weight 358 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC027484
Gene Symbol TRIB3
Gene ID (NCBI) 57761
RRIDAB_10641988
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Protocols

Product Specific Protocols
WB protocol for TRIB3 antibody 13300-1-APDownload protocol
IF protocol for TRIB3 antibody 13300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

J Cachexia Sarcopenia Muscle

Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.

Authors - Guo-Kai Shang
monkeyIHC

Aging Dis

Age-Related Decline in Expression of Molecular Chaperones Induces Endoplasmic Reticulum Stress and Chondrocyte Apoptosis in Articular Cartilage.

Authors - Li Tan
humanWB

Autophagy

Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.

Authors - Tengfei Huang
  • KD Validated
humanWB,IF

Clin Cancer Res

PMID: 31235507

Authors - Jian Lin
  • KD Validated
WB

EMBO Rep

Wnt regulates amino acid transporter Slc7a5 and so constrains the integrated stress response in mouse embryos.

Authors - Nadège Poncet
humanWB

Oxid Med Cell Longev

Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

Authors - Lili Shi
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Chun (Verified Customer) (12-05-2019)

a good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: HEK293 cells
FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

I used this antibody in mouse whole brain lysate and in brain endothelial cells, in both the system they work really well. i would definitely recommend this product.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000 for WB
  • Cell Tissue Type: Mouse brain endothelial cells, mouse brain tissue lysate
TRIB3 Antibody Western Blot, validation (1:1000 for WB dilution) in Mouse brain endothelial cells, mouse brain tissue lysate (Cat no:13300-1-AP)
FH

Jose (Verified Customer) (11-02-2017)

Bright nuclear staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Skleletal muscle