Tested Applications
| Positive WB detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13300-1-AP targets TRIB3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4085 Product name: Recombinant human TRIB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC027484 Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG Predict reactive species |
| Full Name | tribbles homolog 3 (Drosophila) |
| Calculated Molecular Weight | 358 aa, 40 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC027484 |
| Gene Symbol | TRIB3 |
| Gene ID (NCBI) | 57761 |
| RRID | AB_10641988 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| WB protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody against TRIB3 (catalog no. 13300-1-AP) has accumulated 52 citations across journals including Autophagy, Cell Death & Disease, Clinical Cancer Research, EMBO Reports, FASEB Journal, Cancer Letters, and Stem Cell Research & Therapy. Research fields span oncology (hepatocellular, breast, ovarian, endometrial, colorectal, and lung cancers), autophagy, endoplasmic reticulum stress, ferroptosis, metabolic disorders (NAFLD, diabetes, insulin resistance), sarcopenia, cardiovascular disease, neurodegeneration, and fibrosis.
| Species | Application | Title |
|---|---|---|
Clin Mol Hepatol MET promotes hepatocellular carcinoma development through the promotion of TRIB3-mediated FOXO1 degradation
| ||
Autophagy ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis | ||
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
| ||
Cell Death Dis RNA N6-methyladenosine demethylase FTO promotes diabetic wound healing through TRIB3-mediated autophagy in an m6A-YTHDF2-dependent manner | ||
Cell Death Dis TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis |
Reviews
What customers say
Users report positive experiences with this antibody across multiple applications. For Western blot, it performed well on HEK293 cells (1:2,000) and mouse brain tissue/endothelial cell lysates (1:1,000), with one reviewer strongly recommending the product. For immunofluorescence, a user observed bright nuclear staining in skeletal muscle at 1:100 dilution. Overall, reviewers rate it favorably for its reliable performance and clear signal in both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chun (Verified Customer) (12-05-2019) | a good antibody
|
KARTHIKEYAN (Verified Customer) (11-20-2019) | I used this antibody in mouse whole brain lysate and in brain endothelial cells, in both the system they work really well. i would definitely recommend this product.
 |
Jose (Verified Customer) (11-02-2017) | Bright nuclear staining.
|
