TRIB3 Monoclonal antibody

TRIB3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C10C7

Cat no : 66702-1-Ig

Synonyms

C20orf97, NIPK, SINK, SKIP3, TRB 3, TRB3, TRIB3, Tribbles homolog 3



Tested Applications

Positive WB detected inHT-29 cells, MCF-7 cells, rat liver tissue, mouse hepatocytes, DU 145 cells, LNCaP cells, PC-3 cells, HUVEC cells, HepG2 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66702-1-Ig targets TRIB3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIB3 fusion protein Ag25776
Full Name tribbles homolog 3 (Drosophila)
Calculated Molecular Weight 358 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC027484
Gene Symbol TRIB3
Gene ID (NCBI) 57761
RRIDAB_2882055
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Protocols

Product Specific Protocols
WB protocol for TRIB3 antibody 66702-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

JCI Insight

Mitochondrial and NAD+ metabolism predict recovery from acute kidney injury in a diverse mouse population

Authors - Jean-David Morel
humanWB

Drug Des Devel Ther

PMID: 31118581

Authors - Hang Zhao