Tested Applications
| Positive WB detected in | HT-29 cells, MCF-7 cells, rat liver tissue, mouse hepatocytes, DU 145 cells, LNCaP cells, PC-3 cells, HUVEC cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66702-1-Ig targets TRIB3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25776 Product name: Recombinant human TRIB3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-358 aa of BC027484 Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG Predict reactive species |
| Full Name | tribbles homolog 3 (Drosophila) |
| Calculated Molecular Weight | 358 aa, 40 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC027484 |
| Gene Symbol | TRIB3 |
| Gene ID (NCBI) | 57761 |
| RRID | AB_2882055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96RU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIB3 antibody 66702-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIB3 Monoclonal antibody (2C10C7), catalog number 66702-1-Ig, has 5 total citations. These appear in Phytomedicine, Biomed Pharmacother, JCI Insight, Drug Design, Development and Therapy, and Lasers in Medical Science. The associated research fields span gastric cancer autophagy, mesothelioma apoptosis, acute kidney injury metabolism, hepatic endoplasmic reticulum stress and insulin sensitivity, and melasma pigment-clearance mechanisms.
| Species | Application | Title |
|---|---|---|
Phytomedicine Cepharanthine sensitizes gastric cancer cells to chemotherapy by targeting TRIB3-FOXO3-FOXM1 axis to inhibit autophagy | ||
Biomed Pharmacother Curcumin impacts on cisplatin sensitivity determining apoptotic boost in mesothelioma. | ||
JCI Insight Mitochondrial and NAD+ metabolism predict recovery from acute kidney injury in a diverse mouse population | ||
Lasers Med Sci Melanogenesis inhibition and anti-inflammation is essential for pigment-clearance in melasma treated by low-fluence Q-switched nd: YAG 1064 nm laser. |
