TRIB3 Monoclonal antibody

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  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66702-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse hepatocytes using 66702-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 66702-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66702-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TRIB3 Monoclonal Antibody for WB, ELISA

Cat No. 66702-1-Ig

Clone No.2C10C7

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

2C10C7, C20orf97, Neuronal cell death-inducible putative kinase, NIPK, p65-interacting inhibitor of NF-kappa-B

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Tested Applications

• Positive WB detected in: HT-29 cells, MCF-7 cells, rat liver tissue, mouse hepatocytes, DU 145 cells, LNCaP cells, PC-3 cells, HUVEC cells, HepG2 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

66702-1-Ig targets TRIB3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25776

  Product name: Recombinant human TRIB3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-358 aa of BC027484

  Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG

• Full Name: tribbles homolog 3 (Drosophila)
• Calculated Molecular Weight: 358 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC027484
• Gene Symbol: TRIB3
• Gene ID (NCBI): 57761
• RRID: AB_2882055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96RU7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	JCI Insight; Mitochondrial and NAD+ metabolism predict recovery from acute kidney injury in a diverse mouse population; Authors - Jean-David Morel
human	WB	Phytomedicine; Cepharanthine sensitizes gastric cancer cells to chemotherapy by targeting TRIB3-FOXO3-FOXM1 axis to inhibit autophagy; Authors - Yang-Yang Lu
human	WB	Drug Des Devel Ther; PMID: 31118581; Authors - Hang Zhao