TRIM16 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM16 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 24403-1-AP

Synonyms

EBBP, TRIM16, tripartite motif containing 16

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Validation Data Gallery

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  WB analysis of HepG2 using 24403-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24403-1-AP (TRIM16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24403-1-AP

  IP result of anti-TRIM16 (IP:24403-1-AP, 4ug; Detection:24403-1-AP 1:300) with HeLa cells lysate 3960ug.

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 24403-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 24403-1-AP (TRIM16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 24403-1-AP is TRIM16 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM16 fusion protein Ag21406
• Full Name: tripartite motif-containing 16
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC015674
• Gene Symbol: TRIM16
• Gene ID (NCBI): 10626
• RRID: AB_2879525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2.; Authors - Yi Zhao ; KD Validated
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng
human	WB	Exp Cell Res; TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.; Authors - Yi Zhao
human	WB,IHC	Exp Cell Res; TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.; Authors - Longhui Ruan ; KD Validated
human	WB	Cell Prolif; G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration; Authors - Shuai Li
human	WB,IF	Mol Carcinog; TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway; Authors - Shanfeng Li ; KD Validated