TRIM16 Recombinant monoclonal antibody, PBS Only (Capture)

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• TRIM16 Recombinant monoclonal antibody, PBS Only (Capture) (87377-3-PBS)

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  WB analysis using 87377-3-RR (same clone as 87377-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87377-3-RR (TRIM16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87377-3-RR (same clone as 87377-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 87377-3-RR (same clone as 87377-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87377-3-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02988-1

  The mean TRIM16 concentration was determined to be 1654.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 47.9 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02988-1

  Cytometric bead array standard curve of MP02988-1, TRIM16 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87377-3-PBS. Detection antibody: 87377-2-PBS. Standard: Ag21406. Range: 1.562-200 ng/mL.

TRIM16 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 87377-3-PBS

Clone No.252519D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16

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Product Information

87377-3-PBS targets TRIM16 as part of a matched antibody pair:

MP02988-1: 87377-3-PBS capture and 87377-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21406

  Product name: Recombinant human TRIM16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC015674

  Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

• Full Name: tripartite motif-containing 16
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC015674
• Gene Symbol: TRIM16
• Gene ID (NCBI): 10626
• RRID: AB_3745496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95361
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902). Ramos is the negative control.