TRIM16 Recombinant monoclonal antibody

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• TRIM16 Recombinant monoclonal antibody (87377-3-RR)

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  WB analysis using 87377-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87377-3-RR (TRIM16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 87377-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A549 using 87377-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using TRIM16 antibody (87377-3-RR, Clone: 252519D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TRIM16 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87377-3-RR

Clone No.252519D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HaCaT cells
• Positive IF/ICC detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87377-3-RR targets TRIM16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag21406

  Product name: Recombinant human TRIM16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-349 aa of BC015674

  Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP

• Full Name: tripartite motif-containing 16
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC015674
• Gene Symbol: TRIM16
• Gene ID (NCBI): 10626
• RRID: AB_3745496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902). Ramos is the negative control.