Tested Applications
| Positive WB detected in | HEK-293 cells, HCT 116 cells, HT-29 cells, Caco-2 cells, SW480 cells, U-251 cells, H9C2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87196-1-RR targets TRIM38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3840 Product name: Recombinant human TRIM38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 117-466 aa of BC026930 Sequence: RAPQHKGHTTALVEDVCQGYKEKLQKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLSRLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLYFDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFEVDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEAGVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD Predict reactive species |
| Full Name | tripartite motif-containing 38 |
| Calculated Molecular Weight | 465 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC026930 |
| Gene Symbol | TRIM38 |
| Gene ID (NCBI) | 10475 |
| RRID | AB_3745400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00635 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM38 antibody 87196-1-RR | Download protocol |
| IHC protocol for TRIM38 antibody 87196-1-RR | Download protocol |
| WB protocol for TRIM38 antibody 87196-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIM38 Recombinant monoclonal antibody (252271E8), catalog number 87196-1-RR, has 1 total citation. The citation appears in Drug Dev Res (Impact Factor 4.2), published in 2026. The associated research field involves ferroptosis and heart failure, specifically examining how TRIM38 alleviates ferroptosis by inactivating the TRAF6-p38 MAPK pathway through ubiquitinating degradation of IRAK1.
| Species | Application | Title |
|---|---|---|
Drug Dev Res TRIM38 Alleviates Ferroptosis in Heart Failure by Inactivating the TRAF6-p38 MAPK Pathway via Ubiquitinating Degradation of IRAK1. |
