TRIM38 Recombinant monoclonal antibody

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  WB analysis using 87196-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87196-1-RR (TRIM38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 87196-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TRIM38 antibody (87196-1-RR, Clone: 252271E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87196-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87196-1-RR against Human TRIM38 were performed. The affinity constant is below 1 pM.

TRIM38 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87196-1-RR

Clone No.252271E8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM38, EC:2.3.2.27, RING finger protein 15, RNF15, RORET

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Tested Applications

• Positive WB detected in: HEK-293 cells, HCT 116 cells, HT-29 cells, Caco-2 cells, SW480 cells, U-251 cells, H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87196-1-RR targets TRIM38 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3840

  Product name: Recombinant human TRIM38 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 117-466 aa of BC026930

  Sequence: RAPQHKGHTTALVEDVCQGYKEKLQKAVTKLKQLEDRCTEQKLSTAMRITKWKEKVQIQRQKIRSDFKNLQCFLHEEEKSYLWRLEKEEQQTLSRLRDYEAGLGLKSNELKSHILELEEKCQGSAQKLLQNVNDTLSRSWAVKLETSEAVSLELHTMCNVSKLYFDVKKMLRSHQVSVTLDPDTAHHELILSEDRRQVTRGYTQENQDTSSRRFTAFPCVLGCEGFTSGRRYFEVDVGEGTGWDLGVCMENVQRGTGMKQEPQSGFWTLRLCKKKGYVALTSPPTSLHLHEQPLLVGIFLDYEAGVVSFYNGNTGCHIFTFPKASFSDTLRPYFQVYQYSPLFLPPPGD

• Full Name: tripartite motif-containing 38
• Calculated Molecular Weight: 465 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC026930
• Gene Symbol: TRIM38
• Gene ID (NCBI): 10475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00635
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549).