TRIM46 Polyclonal antibody, PBS Only

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• TRIM46 Polyclonal antibody, PBS Only (21026-1-PBS)

• 

  WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21026-1-AP (same clone as 21026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (21026-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 21026-1-AP (same clone as 21026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (21026-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 21026-1-AP (same clone as 21026-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (21026-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 21026-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRIM46 Polyclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA

Cat No. 21026-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Synonyms

GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46, Tripartite, fibronectin type-III and C-terminal SPRY motif protein

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Product Information

21026-1-PBS targets TRIM46 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15290

  Product name: Recombinant human TRIM46 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC069416

  Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI

• Full Name: tripartite motif-containing 46
• Calculated Molecular Weight: 759 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC069416
• Gene Symbol: TRIM46
• Gene ID (NCBI): 80128
• RRID: AB_10732843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z4K8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).