TRIM62 Polyclonal antibody, PBS Only

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• TRIM62 Polyclonal antibody, PBS Only (15256-1-PBS)

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  WB analysis of MCF-7 using 15256-1-AP (same clone as 15256-1-PBS)

  WB result of TRIM62 antibody (15256-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM62 transfected MCF-7 cells. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  WB analysis using 15256-1-AP (same clone as 15256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15256-1-AP (same clone as 15256-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15256-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase TRIM62, EC:2.3.2.27, tripartite motif containing 62, Tripartite motif-containing protein 62

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Product Information

15256-1-PBS targets TRIM62 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7143

  Product name: Recombinant human TRIM62 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 126-475 aa of BC001222

  Sequence: TGIDDAFDELQRELKDQLQALQDSEREHTEALQLLKRQLAETKSSTKSLRTTIGEAFERLHRLLRERQKAMLEELEADTARTLTDIEQKVQRYSQQLRKVQEGAQILQERLAETDRHTFLAGVASLSERLKGKIHETNLTYEDFPTSKYTGPLQYTIWKSLFQDIHPVPAALTLDPGTAHQRLILSDDCTIVAYGNLHPQPLQDSPKRFDVEVSVLGSEAFSSGVHYWEVVVAEKTQWVIGLAHEAASRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVRDKLDKVGVFLDYDQGLLIFYNADDMSWLYTFREKFPGKLCSYFSPGQSHANGKNVQPLRINTVRI

• Full Name: tripartite motif-containing 62
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC001222
• Gene Symbol: TRIM62
• Gene ID (NCBI): 55223
• RRID: AB_3669202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BVG3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).