TRIM63 Recombinant antibody

• 

  WB analysis using 82345-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82345-4-RR (TRIM63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TRIM63 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 82345-4-RR

Clone No.242055A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MuRF1, MuRF 1, Iris RING finger protein, IRF, EC:2.3.2.27

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Tested Applications

• Positive WB detected in: rat skeletal muscle tissue, mouse skeletal muscle

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82345-4-RR targets TRIM63 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: tripartite motif-containing 63
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: NM_032588
• Gene Symbol: TRIM63
• Gene ID (NCBI): 84676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q969Q1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM63, also named as IRF, MURF1, RNF28 and SMRZ, is E3 ubiquitin ligase. It is regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. TRIM63 may play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. It may regulate the organization of myofibrils through TTN in muscle cells. TRIM63 is in muscle-specific expression.