TRIM72 Monoclonal antibody, PBS Only

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  WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67403-1-Ig (same clone as 67403-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Cat No. 67403-1-PBS

Clone No.5F2C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Synonyms

hMG53, Mg53, Mitsugumin 53, Mitsugumin-53, TRIM

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Product Information

67403-1-PBS targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2560

  Product name: Recombinant human TRIM72 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 145-477 aa of BC033211

  Sequence: MRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA

• Full Name: tripartite motif-containing 72
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033211
• Gene Symbol: TRIM72
• Gene ID (NCBI): 493829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q6ZMU5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.