TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-Ig

Synonyms

hMG53, Mg53, Mitsugumin 53, TRIM72, tripartite motif containing 72

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Validation Data Gallery

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  WB analysis using 67403-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 67403-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 67403-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67403-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of C2C12 using 67403-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
• Positive IF detected in: mouse skeletal muscle tissue
• Positive FC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67403-1-Ig targets TRIM72 in WB, IF, FC, ELISA applications and shows reactivity with Human, pig, mouse samples.

• Tested Reactivity: Human, pig, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM72 fusion protein Ag2560
• Full Name: tripartite motif-containing 72
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033211
• Gene Symbol: TRIM72
• Gene ID (NCBI): 493829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.