TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Cat No. 67403-1-Ig
Clone No.5F2C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

hMG53, Mg53, Mitsugumin 53, Mitsugumin-53, TRIM

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67403-1-Ig targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM72 fusion protein Ag2560 Predict reactive species
Full Name tripartite motif-containing 72
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033211
Gene Symbol TRIM72
Gene ID (NCBI) 493829
RRIDAB_3085040
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ6ZMU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRIM72 antibody 67403-1-IgDownload protocol
IF protocol for TRIM72 antibody 67403-1-IgDownload protocol
FC protocol for TRIM72 antibody 67403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

MG53 deficiency mediated skeletal muscle dysfunction in chronic obstructive pulmonary disease via impairing mitochondrial fission

Authors - Liwei Liao
  • KO Validated
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