TRMU Recombinant antibody

• 

  WB analysis using 86095-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86095-1-RR (TRMU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86095-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86095-1-RR against Human TRMU were performed. The affinity constant is below 1 pM.

TRMU Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86095-1-RR

Clone No.250434A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

Synonyms

EC:2.8.1.14, Mitochondrial tRNA-specific 2-thiouridylase 1, MTO2, MTO2 homolog, MTU1

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, A549 cells, MCF-7 cells, U2OS cells, NIH/3T3 cells, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86095-1-RR targets TRMU in applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRMU fusion protein Ag21930
• Full Name: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
• Calculated Molecular Weight: 421 aa, 48 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC027991
• Gene Symbol: TRNT1
• Gene ID (NCBI): 55687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75648
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRMU (tRNA m5C methyltransferase) is a mitochondrial protein involved in the modification of mitochondrial tRNA. Specifically, it catalyzes the formation of 5-methylcytidine at position 34 (m5C34) in the anticodon stem-loop of mitochondrial tRNAs, which is crucial for maintaining the stability and function of these tRNAs. TRMU is a nuclear-encoded protein located in the mitochondrial matrix. It plays a vital role in mitochondrial translation and oxidative phosphorylation.TRMU is associated with several genetic disorders. Mutations in the TRMU gene can cause mitochondrial diseases, such as infantile hepatic failure syndrome. (PMID:23625533)