TRPC3 Recombinant antibody

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  WB analysis of HEK-293 using 84019-5-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84019-5-RR (TRPC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84019-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84019-5-RR (TRPC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TRPC3 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84019-5-RR

Clone No.241075H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Transient receptor protein 3, Short transient receptor potential channel 3, hTrp-3, hTrp3, hTrp 3

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, HEK-293 cells, 37℃ incubated mouse brain tissue, unboiled rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84019-5-RR targets TRPC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRPC3 fusion protein Ag13024
• Full Name: transient receptor potential cation channel, subfamily C, member 3
• Calculated Molecular Weight: 848 aa, 97 kDa
• Observed Molecular Weight: 105 kDa, 97 kDa
• GenBank Accession Number: BC093682
• Gene Symbol: TRPC3
• Gene ID (NCBI): 7222
• RRID: AB_3671588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13507
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPC3 (Short transient receptor potential channel 3), also known as TRP3. It is expected to be located in the cell membrane, which is expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis. TRPC3 is one of the classical members of the mammalian transient receptor potential (TRP) superfamily of ion channels. TRPC3 is a molecule with intriguing sensory features including the direct recognition of and activation by diacylglycerols (DAG). Although TRPC3 channels are ubiquitously expressed, they appear to control functions of the cardiovascular system and the brain in a highly specific manner. Moreover, a role of TRPC3 in immunity, cancer, and tissue remodeling has been proposed, generating much interest in TRPC3 as a target for pharmacological intervention. The molecular weight of TRPC3 is 105 kDa, and it can recognize another isoform. (PMID: 30037143)