TRPC6 Polyclonal antibody, PBS Only

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• TRPC6 Polyclonal antibody, PBS Only (18236-1-PBS)

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  WB analysis of mouse brain using 18236-1-AP (same clone as 18236-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  WB analysis using 18236-1-AP (same clone as 18236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 18236-1-AP (same clone as 18236-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 18236-1-AP (same clone as 18236-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 18236-1-AP (same clone as 18236-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 18236-1-AP (same clone as 18236-1-PBS)

  IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 18236-1-AP (same clone as 18236-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TRPC6 antibody (18236-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 18236-1-AP (same clone as 18236-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 18236-1-AP (same clone as 18236-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 18236-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 18236-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

FSGS2, Transient receptor protein 6, TRP 6, TRP6, TRP-6

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Product Information

18236-1-PBS targets TRPC6 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12976

  Product name: Recombinant human TRPC6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-405 aa of BC093658

  Sequence: MSQSPAFGPRRGSSPRGAAGAAARRNESQDYLLMDSELGEDGCPQAPLPCYGYYPCFRGSDNRLAHRRQTVLREKGRRLANRGPAYMFSDRSTSLSIEEERFLDAAEYGNIPVVRKMLEECHSLNVNCVDYMGQNALQLAVANEHLEITELLLKKENLSRVGDALLLAISKGYVRIVEAILSHPAFAEGKRLATSPSQSELQQDDFYAYDEDGTRFSHDVTPIILAAHCQEYEIVHTLLRKGARIERPHDYFCKCNDCNQKQKHDSFSHSRSRINAYKGLASPAYLSLSSEDPVMTALELSNELAVLANIEKEFKNDYKKLSMQCKDFVVGLLDLCRNTEEVEAILNGDVETLQSGDHGRPNLSRLKLAIKYEVKKFVAHPNCQQQLLSIWYENLSGLRQQTMAV

• Full Name: transient receptor potential cation channel, subfamily C, member 6
• Calculated Molecular Weight: 931 aa, 106 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC093658
• Gene Symbol: TRPC6
• Gene ID (NCBI): 7225
• RRID: AB_10859822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y210
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).