TRPV1 Polyclonal antibody

TRPV1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22686-1-AP

Synonyms

Capsaicin receptor, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1



Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inhuman skin tissue, mouse skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22686-1-AP is TRPV1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPV1 fusion protein Ag18583
Full Name transient receptor potential cation channel, subfamily V, member 1
Calculated molecular weight 839 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC132820
Gene symbol TRPV1
Gene ID (NCBI) 7442
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Protocols

Product Specific Protocols
WB protocol for TRPV1 antibody 22686-1-APDownload protocol
IHC protocol for TRPV1 antibody 22686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Pharmacol Sin

PMID: 29188798

Authors - Su-Shan Zhang
ratIHC

Cell Death Dis

TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway.

Authors - Zhongyang Lv
humanIF

Acta Otolaryngol

Expressions of TRPVs in the cholesteatoma epithelium.

Authors - Ba Hung Do
mouseWB

J Agric Food Chem

Gingerol Reverses the Cancer-Promoting Effect of Capsaicin by Increased TRPV1 Level in a Urethane-Induced Lung Carcinogenic Model.

Authors - Shengnan Geng
mouseWB

Eur J Pharmacol

Spinophilin negatively controlled the function of transient receptor potential vanilloid 1 in dorsal root ganglia neurons of mice.

Authors - Zhen Guo
ratWB

Biomed Pharmacother

Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.

Authors - Weiwei Qin