Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, mouse brain tissue, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|mouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:400-1:1600
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
24601-1-AP targets Tuberin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse , rat samples.
|human, mouse , rat
|Host / Isotype
|Rabbit / IgG
|Tuberin fusion protein Ag18133
|tuberous sclerosis 2
|Calculated molecular weight
|1807 aa, 201 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.
|Product Specific Protocols
|WB protocol for Tuberin antibody 24601-1-AP
|IHC protocol for Tuberin antibody 24601-1-AP
|IF protocol for Tuberin antibody 24601-1-AP
|IP protocol for Tuberin antibody 24601-1-AP
|Click here to view our Standard Protocols
Eur Rev Med Pharmacol Sci
Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.
Oleanolic Acid Induces Autophagy and Apoptosis via the AMPK-mTOR Signaling Pathway in Colon Cancer.
NPJ Breast Cancer
Resistance to anti-HER2 therapy associated with the TSC2 nonsynonymous variant c.4349 C > G (p.Pro1450Arg) is reversed by CDK4/6 inhibitor in HER2-positive breast cancer
A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression
Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate
Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma