Tuberin Polyclonal antibody

Tuberin Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IP, IF

Conjugate

Unconjugated

Cat no : 24601-1-AP

Synonyms

LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 24601-1-AP is Tuberin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tuberin fusion protein Ag18133
Full Name tuberous sclerosis 2
Calculated molecular weight 1807 aa, 201 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC150300
Gene symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Tuberin antibody 24601-1-APDownload protocol
IHC protocol for Tuberin antibody 24601-1-APDownload protocol
IF protocol for Tuberin antibody 24601-1-APDownload protocol
IP protocol for Tuberin antibody 24601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Oncol

Oleanolic Acid Induces Autophagy and Apoptosis via the AMPK-mTOR Signaling Pathway in Colon Cancer.

Authors - Changxiao Hu
humanWB

Eur Rev Med Pharmacol Sci

Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.

Authors - X-L Zhang