Tuberin Polyclonal antibody

Tuberin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 24601-1-AP

Synonyms

LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

24601-1-AP targets Tuberin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tuberin fusion protein Ag18133
Full Name tuberous sclerosis 2
Calculated molecular weight 1807 aa, 201 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC150300
Gene symbol TSC2
Gene ID (NCBI) 7249
RRIDAB_2879633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.

Protocols

Product Specific Protocols
WB protocol for Tuberin antibody 24601-1-APDownload protocol
IHC protocol for Tuberin antibody 24601-1-APDownload protocol
IF protocol for Tuberin antibody 24601-1-APDownload protocol
IP protocol for Tuberin antibody 24601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur Rev Med Pharmacol Sci

Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.

Authors - X-L Zhang
humanWB

J Oncol

Oleanolic Acid Induces Autophagy and Apoptosis via the AMPK-mTOR Signaling Pathway in Colon Cancer.

Authors - Changxiao Hu
humanWB

NPJ Breast Cancer

Resistance to anti-HER2 therapy associated with the TSC2 nonsynonymous variant c.4349 C > G (p.Pro1450Arg) is reversed by CDK4/6 inhibitor in HER2-positive breast cancer

Authors - Ziyan Yang
humanWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
humanWB

Autophagy

Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate

Authors - Mengting Ou
humanWB

Transl Res

Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma

Authors - Mingyang Li