Tuberin Polyclonal antibody

Tuberin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24601-1-AP

Synonyms

LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

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Validation Data Gallery

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  WB analysis using 24601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24601-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 24601-1-AP

  IP result of anti-Tuberin(IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug.

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  IHC staining of mouse brain using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 24601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 24601-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin antibody (24601-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 24601-1-AP is Tuberin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse , rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tuberin fusion protein Ag18133
• Full Name: tuberous sclerosis 2
• Calculated molecular weight: 1807 aa, 201 kDa
• Observed molecular weight: 200 kDa
• GenBank accession number: BC150300
• Gene symbol: TSC2
• Gene ID (NCBI): 7249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	J Oncol; Oleanolic Acid Induces Autophagy and Apoptosis via the AMPK-mTOR Signaling Pathway in Colon Cancer.; Authors - Changxiao Hu
human	WB	NPJ Breast Cancer; Resistance to anti-HER2 therapy associated with the TSC2 nonsynonymous variant c.4349 C > G (p.Pro1450Arg) is reversed by CDK4/6 inhibitor in HER2-positive breast cancer; Authors - Ziyan Yang