TSEN2 Polyclonal antibody

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TSEN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 13103-2-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

EC:4.6.1.16, HsSen2, PCH2B, SEN2, SEN2L

• 

  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse colon using 13103-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 13103-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 13103-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 13103-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TSEN2 antibody (13103-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse colon tissue, human brain tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13103-2-AP targets TSEN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3742

  Product name: Recombinant human TSEN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 159-465 aa of BC019582

  Sequence: SNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFRSKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELMLCYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL

• Full Name: tRNA splicing endonuclease 2 homolog (S. cerevisiae)
• Calculated Molecular Weight: 465 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC019582
• Gene Symbol: TSEN2
• Gene ID (NCBI): 80746
• RRID: AB_2272213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCE0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).

Publications

What published studies show

The TSEN2 Polyclonal antibody (Cat# 13103-2-AP) has 3 citations published in Cell, American Journal of Human Genetics, and Genetics. The research spans tRNA splicing and maturation, cerebellar development, neurodegeneration, pontocerebellar hypoplasia, progressive microcephaly, integrated stress response, and intron debranching. All citations used the antibody for Western blot in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.; Authors - Ashleigh E Schaffer
human	WB	Am J Hum Genet; Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly.; Authors - Martin W Breuss
human	WB	Genetics; An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching; Authors - Jennifer E Hurtig ; KD Validated