TSGA10 Polyclonal antibody

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• TSGA10 Polyclonal antibody (12593-1-AP)

TSGA10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12593-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

CEP4L, testis specific, 10, TSGA10

• 

  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat testis using 12593-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 12593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 12593-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12593-1-AP targets TSGA10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3311

  Product name: Recombinant human TSGA10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 394-698 aa of BC028366

  Sequence: LEVNKLKNILKSEESENRQMMEQLRKANEDAENWENKARQSEADNNTLKLELITAEAEGNRLKEKVDSLNREVEQHLNAERSYKSQISTLHKSVVKMEEELQKVQFEKVSALADLSSTRELCIKLDSSKELLNRQLVAKDQEIEMRENELDSAHSEIELLRSQMANERISMQNLEALLVANRDKEYQSQIALQEKESEIQLLKEHLCLAENKMAIQSRDVAQFRNVVTQLEADLDITKRQLGTERFERERAVQELRRQNYSSNAYHMSSTMKPNTKCHSPERAHHRSPDRGLDRSLEENLCYRDF

• Full Name: testis specific, 10
• Calculated Molecular Weight: 698 aa, 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC028366
• Gene Symbol: TSGA10
• Gene ID (NCBI): 80705
• RRID: AB_2240967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TSGA10 Polyclonal Antibody (Cat# 12593-1-AP) has 7 total citations. These appear in Frontiers in Cell and Developmental Biology, Journal of Cellular and Molecular Medicine, Journal of Assisted Reproduction and Genetics, Basic and Clinical Andrology, and Clinical Genetics. The research fields primarily involve acephalic spermatozoa syndrome and reproductive biology, with additional applications in bone formation and osteoporosis. Cited applications include WB, IF, and IHC in human and mouse samples.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome.; Authors - Yanwei Sha
mouse	WB,IHC	Front Cell Dev Biol; miR-129-5p Inhibits Bone Formation Through TCF4.; Authors - Chong Yin
human,mouse	WB，IHC	J Cell Mol Med; MACF1 alleviates aging-related osteoporosis via HES1.; Authors - Chong Yin
human	WB	J Assist Reprod Genet; Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10.; Authors - Mingfei Xiang
human	WB,IF	Basic Clin Androl; A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family; Authors - Khalid Khan
human,mouse	IF	Clin Genet; Biallelic mutations in PMFBP1 cause acephalic spermatozoa.; Authors - Yan-Wei Sha