TTLL12 Polyclonal antibody, PBS Only

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• TTLL12 Polyclonal antibody, PBS Only (21447-1-PBS)

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  WB analysis using 21447-1-AP (same clone as 21447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21447-1-AP (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21447-1-AP (TTLL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 21447-1-AP (same clone as 21447-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTLL12 antibody (21447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21447-1-PBS in a different storage buffer formulation.

TTLL12 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 21447-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Inactive tubulin--tyrosine ligase-like protein 12, Tubulin--tyrosine ligase-like protein 12

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Product Information

21447-1-PBS targets TTLL12 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13804

  Product name: Recombinant human TTLL12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 293-644 aa of BC001070

  Sequence: DINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTLFLDQPGGCHVTCLV

• Full Name: tubulin tyrosine ligase-like family, member 12
• Calculated Molecular Weight: 644 aa, 74 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001070
• Gene Symbol: TTLL12
• Gene ID (NCBI): 23170
• RRID: AB_3669368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14166
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).