Alpha Tubulin Polyclonal antibody
Alpha Tubulin Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14555-1-AP
Synonyms
"Alpha Tubulin Antibodies" Comparison
View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | mouse brain tissue, mouse cerebellum tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
14555-1-AP targets Alpha Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha Tubulin fusion protein Ag6038 |
Full Name | tubulin, alpha 1a |
Calculated molecular weight | 50 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC050637 |
Gene symbol | TUBA1A |
Gene ID (NCBI) | 7846 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
IHC protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
IF protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Neurosci Res Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion. | ||
J Cell Sci ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments. | ||
Biochim Biophys Acta Mol Basis Dis Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E. | ||
Exp Cell Res Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. | ||
Cell Rep SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation. | ||
Nanomedicine Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer. |