"Alpha Tubulin Antibodies" Comparison
View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse cerebellum tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue, rat kidney tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6038 Product name: Recombinant human tubulin-Alpha protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-451 aa of BC050637 Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY Predict reactive species |
| Full Name | tubulin, alpha 1a |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC050637 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2212258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q71U36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| IHC protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| WB protocol for Alpha Tubulin antibody 14555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alpha Tubulin Polyclonal antibody (catalog no. 14555-1-AP) has 82 citations. They appear in high-impact journals including Cell, Nature Communications, Science Advances, Cell Reports, Cell Death & Disease, eLife, Oncogene, and Molecular Cancer. Research fields span oncology (cancer metabolism, ferroptosis, DNA damage repair), neuroscience (Parkinson's, tau, sleep), immunology/inflammation, virology, metabolism, reproductive biology, and fibrosis, with Western blot as the dominant application.
| Species | Application | Title |
|---|---|---|
Cell Rift Valley fever virus coordinates the assembly of a programmable E3 ligase to promote viral replication | ||
Mol Cancer Inhibition of PRMT5 triggers synthetic lethality in ARID1A-deficient endometrial cancer by promoting aberrant R-loop accumulation. | ||
Cell Metab Uridine depletion impairs CD8⁺ T cell antitumor activity through N-glycosylation. | ||
J Extracell Vesicles M2 Macrophage-Derived Migrasomes Mediate Ischaemia-Induced Retinal Neovascularization by Targeting TREM2. | ||
Nat Commun Structural and evolutionary insights into the eukaryotic RNase MRP ribonucleoprotein complex. | ||
Nat Commun Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice |
Reviews
What customers say
Both reviews rate the product 5 stars for Western Blot applications. One user tested it on KMS11 cells at a 1:5000 dilution and described it as a "great antibody for western blot." The other user tested it on human samples and simply called it a "good product." Overall, customers express high satisfaction with its performance in WB, with no negative feedback reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
lu (Verified Customer) (12-04-2025) | Great antibody for western blot
|
Yuting (Verified Customer) (08-27-2025) | Good product
|
