Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10063-2-AP targets Beta Tubulin in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0108 Product name: Recombinant human tubulin-beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-266 aa of BC001352 Sequence: QCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF Predict reactive species |
| Full Name | tubulin, beta 2B |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50kda |
| GenBank Accession Number | BC001352 |
| Gene Symbol | Beta Tubulin |
| Gene ID (NCBI) | 347733 |
| RRID | AB_2210536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are five tubulins in human cells. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Beta Tubulin antibody 10063-2-AP | Download protocol |
| WB protocol for Beta Tubulin antibody 10063-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (catalog #10063-2-AP) has 12 citations published in journals including Immunity, Bioactive Materials, Nucleic Acids Research, Cancer Biology & Medicine, Advanced Healthcare Materials, Biological Psychiatry, Science of the Total Environment, Journal of the American Society of Nephrology, Ecotoxicology and Environmental Safety, Acta Physiologica, Journal of Biological Chemistry, and Journal of Molecular Neuroscience. Associated research fields span cancer metastasis, bone regeneration, ER biology, prostate cancer, neuronal migration, toxicology, acute kidney injury, DNA damage, hypoxia, and epilepsy.
| Species | Application | Title |
|---|---|---|
Immunity Palmitic acid reprograms neutrophils to compromise vascular integrity and promote breast cancer lung metastasis. | ||
Bioact Mater Copper doped bioactive glass promotes matrix vesicles-mediated biomineralization via osteoblast mitophagy and mitochondrial dynamics during bone regeneration | ||
Nucleic Acids Res Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum. | ||
Cancer Biol Med IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation. | ||
Adv Healthc Mater Magnesium-Impregnated Membrane Promotes Bone Regeneration in Rat Skull Defect by N-Linked Glycosylation of SPARC via MagT1 | ||
Biol Psychiatry Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins. |
