Validation Data Gallery
|Positive WB detected in||HeLa cells, Transfected HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE cells , MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
15176-1-AP targets Gamma Tubulin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
|Tested Reactivity||human, mouse, rat, dog|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Tubulin fusion protein Ag7070|
|Full Name||tubulin, gamma 1|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC000619|
|Gene ID (NCBI)||7283|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
|Product Specific Protocols|
|WB protocol for Gamma Tubulin antibody 15176-1-AP||Download protocol|
|IHC protocol for Gamma Tubulin antibody 15176-1-AP||Download protocol|
|IF protocol for Gamma Tubulin antibody 15176-1-AP||Download protocol|
|IP protocol for Gamma Tubulin antibody 15176-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hum Mol Genet
Nearly complete deletion of BubR1 causes microcephaly through shortened mitosis and massive cell death.
J Cell Sci
Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.
LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.
Cell Death Differ
Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.
Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.
Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christopher (Verified Customer) (07-02-2019)
Very fast delivery I think it arrived in a matter of days which is quick compared to some other companies. Westerns worked really well, so it must be good, over all really pleased with this purchase
Boyan (Verified Customer) (01-29-2019)
Excellent. Works quite well both for WB and IF.