TUBGCP2 Polyclonal antibody

TUBGCP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25856-1-AP

Synonyms

GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2

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Validation Data Gallery

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  WB analysis using 25856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25856-1-AP (TUBGCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25856-1-AP

  IP result of anti-TUBGCP2 (IP:25856-1-AP, 4ug; Detection:25856-1-AP 1:500) with HeLa cells lysate 3920 ug.

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  IHC staining of mouse testis using 25856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 25856-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, U2OS cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

25856-1-AP targets TUBGCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TUBGCP2 fusion protein Ag23066
• Full Name: tubulin, gamma complex associated protein 2
• Calculated Molecular Weight: 902 aa, 103 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC093770
• Gene Symbol: TUBGCP2
• Gene ID (NCBI): 10844
• RRID: AB_2880271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).

Publications

Species	Application	Title
human	IF	Mol Cell Proteomics; Interactome analysis reveals a link of the novel ALMS1-CEP70 complex to centrosomal clusters; Authors - Franziska Woerz