TUFM Polyclonal antibody

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• TUFM Polyclonal antibody (26730-1-AP)

TUFM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 26730-1-AP Copied!

Publications (13)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Synonyms

COXPD4, EF Tu, EF TuMT, EFTU, EF-Tu

• 

  WB analysis using 26730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26730-1-AP (TUFM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 26730-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26730-1-AP (TUFM antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 26730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26730-1-AP (TUFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 26730-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUFM (26730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26730-1-AP targets TUFM in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24981

  Product name: Recombinant human TUFM protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 108-455 aa of BC010041

  Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

• Full Name: Tu translation elongation factor, mitochondrial
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 46-50 kDa
• GenBank Accession Number: BC010041
• Gene Symbol: TUFM
• Gene ID (NCBI): 7284
• RRID: AB_2880616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49411
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Publications

What published studies show

The TUFM Polyclonal antibody (Cat# 26730-1-AP) has 13 citations published in journals including Nature Cell Biology, Nature Communications, Autophagy, Nucleic Acids Research, Clinical Cancer Research, Cell Reports, Journal of Hazardous Materials, Science of the Total Environment, Apoptosis, International Journal of Molecular Medicine, and Ecotoxicology and Environmental Safety. Research fields span mitochondrial translation, mitophagy, cardiomyopathy, neurotoxicity, glioma, osteoarthritis, leukemia, acute pancreatitis, and viral infection.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
human	WB,IF,CoIP	Autophagy; Bunyavirus SFTSV nucleoprotein exploits tufm-mediated mitophagy to impair antiviral innate immunity; Authors - Wen-Kang Zhang
human	WB	Nat Commun; Structural basis of TACO1-mediated efficient mitochondrial translation.; Authors - Shuhui Wang
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
zebrafish	WB	Nucleic Acids Res; Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.; Authors - Danni Chen