TUFM Monoclonal antibody, PBS Only

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  WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

  IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

TUFM Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 67802-1-PBS

Clone No.2A10D2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

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Product Information

67802-1-PBS targets TUFM in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24976

  Product name: Recombinant human TUFM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 108-455 aa of BC010041

  Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

• Full Name: Tu translation elongation factor, mitochondrial
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC010041
• Gene Symbol: TUFM
• Gene ID (NCBI): 7284
• RRID: AB_2918565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49411
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.