TULP1 Polyclonal antibody

TULP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18971-1-AP

Synonyms

TUBL1, Tubby-related protein 1, Tubby-like protein 1

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Validation Data Gallery

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  WB analysis of human brain using 18971-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18971-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18971-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human eye using 18971-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human eye using 18971-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells, human brain tissue, mouse retina tissue
• Positive IHC detected in: human eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18971-1-AP targets TULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TULP1 fusion protein Ag5598
• Full Name: tubby like protein 1
• Calculated Molecular Weight: 489 aa, 55 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC032714
• Gene Symbol: TULP1
• Gene ID (NCBI): 7287
• RRID: AB_2211412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TULP1, also known as RP14, LCA15, and TUBL1, is a member of the tubby-like protein (TULP) family. TULP1 is expressed exclusively in the retina and is essential for the transport of rhodopsin from its site of synthesis in the inner segments through the connecting cilium to the outer segments(PMID: 17620573). Mutations in TULP1 are associated with Retinitis pigmentosa 14 and Leber congenital amaurosis-15(PMID: 17620573, 17962469).

Publications

Species	Application	Title
mouse	WB	Sci Rep; Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation; Authors - Mingzhu Yang