TWF1 Monoclonal antibody, PBS Only

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  WB analysis using 67796-1-Ig (same clone as 67796-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67796-1-Ig (same clone as 67796-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67796-1-Ig (same clone as 67796-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TWF1 antibody (67796-1-Ig, Clone: 2F10F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

TWF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67796-1-PBS

Clone No.2F10F11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

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Product Information

67796-1-PBS targets TWF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28630

  Product name: Recombinant human TWF1 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-342 aa of BC022344

  Sequence: ASEDVKEIFARARNGKYRLLKISIENEQLVIGSYSQPSDSWDKDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD

• Full Name: twinfilin, actin-binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 368 aa, 42 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC022344
• Gene Symbol: TWF1
• Gene ID (NCBI): 5756
• RRID: AB_2918560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12792
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Twinfilin-1 (TWF1) is an actin-binding protein implicated in cell motility, invasion and migration. Its expression is linked to sensitivity to drugs and cancer progression. Overexpression of TWF1 has been reported in various cancers, including breast cancer and lung cancer.