TWF1 Monoclonal antibody

TWF1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2F10F11

Cat no : 67796-1-Ig

Synonyms

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

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Validation Data Gallery

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  WB analysis using 67796-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of Jurkat using 67796-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67796-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TWF1 antibody (67796-1-Ig, Clone: 2F10F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67796-1-Ig targets TWF1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TWF1 fusion protein Ag28630
• Full Name: twinfilin, actin-binding protein, homolog 1 (Drosophila)
• Calculated Molecular Weight: 368 aa, 42 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC022344
• Gene Symbol: TWF1
• Gene ID (NCBI): 5756
• RRID: AB_2918560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Twinfilin-1 (TWF1) is an actin-binding protein implicated in cell motility, invasion and migration. Its expression is linked to sensitivity to drugs and cancer progression. Overexpression of TWF1 has been reported in various cancers, including breast cancer and lung cancer.