Filter: AllWBIHC

WB analysis of HeLa using 22784-1-AP (same clone as 22784-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22784-1-PBS in a different storage buffer formulation.
WB analysis of THP-1 using 22784-1-AP (same clone as 22784-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22784-1-PBS in a different storage buffer formulation.
WB analysis using 22784-1-AP (same clone as 22784-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22784-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 22784-1-AP (same clone as 22784-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22784-1-AP (TWISTNB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22784-1-PBS in a different storage buffer formulation.

TWISTNB Polyclonal antibody, PBS Only

TWISTNB Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 22784-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

22784-1-PBS targets TWISTNB in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18783 Product name: Recombinant human TWISTNB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC130298 Sequence: MAAGCSEAPRPAAASDGSLVGQAGVLPCLELPTYAAACALVNSRYSCLVAGPHQRHIALSPRYLNRKRTGIREQLDAELLRYSESLLGVPIAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSSSHIGCLVHGCFNASIPKPEQLSAEQWQTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNITSLQFKRSEVSEEVTENGTEEAAKKPKKKKKKKDPETYEVDSGTTKLADDADDTPMEESALQNTNNANGIWEEEPKKKKKKKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAE Predict reactive species
Full Name	TWIST neighbor
Calculated Molecular Weight	338 aa, 37 kDa
Observed Molecular Weight	40-50 kDa
GenBank Accession Number	BC130298
Gene Symbol	TWISTNB
Gene ID (NCBI)	221830
RRID	AB_2879167
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3B726
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.