TXNDC15 Polyclonal antibody

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• TXNDC15 Polyclonal antibody (32874-1-AP)

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  WB analysis of HeLa using 32874-1-AP

  WB result of TXNDC15 antibody (32874-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC15 transfected HeLa cells.

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  WB analysis using 32874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32874-1-AP (TXNDC15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 32874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 32874-1-AP (TXNDC15 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TXNDC15 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32874-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C5orf14, Thioredoxin Domain Containing 15, Thioredoxin domain-containing protein 15, UNQ335/PRO534

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, RKO cells, fetal human brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32874-1-AP targets TXNDC15 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37138

  Product name: Recombinant human TXNDC15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 33-321 aa of BC032568

  Sequence: VEVAEESGRLWSEEQPAHPLQVGAVYLGEEELLHDPMGQDRAAEEANAVLGLDTQGDHMVMLSVIPGEAEDKVSSEPSGVTCGAGGAEDSRCNVRESLFSLDGAGAHFPDREEEYYTEPEVAESDAAPTEDSNNTESLKSPKVNCEERNITGLENFTLKILNMSQDLMDFLNPNGSDCTLVLFYTPWCRFSASLAPHFNSLPRAFPALHFLALDASQHSSLSTRFGTVAVPNILLFQGAKPMARFNHTDRTLETLKIFIFNQTGIEAKKNVVVTQADQIGPLPSTLIKS

• Full Name: thioredoxin domain containing 15
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032568
• Gene Symbol: TXNDC15
• Gene ID (NCBI): 79770
• RRID: AB_3742773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96J42
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC15 (thioredoxin domain-containing protein 15) is a single-pass type I transmembrane protein that contains a thioredoxin domain and is primarily localized in the cytoplasm. It plays a positive regulatory role in the Hedgehog signaling pathway within cilia and actively participates in the process of ciliogenesis, exerting significant influence on the structure and function of cilia-an essential cellular organelle. Additionally, TXNDC15 has been found to form mixed disulfide complexes with endoplasmic reticulum-resident protein disulfide isomerase (PDI) family members, such as ERp57, PDI, and ERp44, suggesting its involvement in protein folding and quality control processes. Notably, mutations in the TXNDC15 gene are associated with a rare and lethal genetic disorder known as Meckel-Gruber syndrome, which is caused by defects in ciliogenesis.