CoraLite® Plus 488-conjugated TXNDC5 Recombinant monoclonal antibody

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  IF Staining of U2OS using CL488-84756-5

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TXNDC5 antibody (CL488-84756-5, Clone: 242195G7 ) at dilution of 1:200, CL594-phalloidin (red).

TXNDC5 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84756-5

Clone No.242195G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242195G7, EC:1.8.4.-, EndoPDI, ER protein 46, ERp46

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84756-5 targets TXNDC5 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13965

  Product name: Recombinant human TXNDC5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-324 aa of BC001199

  Sequence: MEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKDEL

• Full Name: thioredoxin domain containing 5 (endoplasmic reticulum)
• Calculated Molecular Weight: 432 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001199
• Gene Symbol: TXNDC5
• Gene ID (NCBI): 81567
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NBS9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TXNDC5, or Thioredoxin Domain Containing 5, is a protein-coding gene that plays a significant role in cellular processes, particularly in the endoplasmic reticulum (ER). This gene is essential for the proper functioning of the cellular environment, primarily through its involvement in the formation and rearrangement of disulfide bonds within proteins. Disulfide bonds are crucial for the stability and functionality of many proteins, and TXNDC5 facilitates these processes through its thioredoxin domains, which contain enzymatic activities that promote correct protein folding and maturation in the ER. Recent studies highlight the expression characteristics of TXNDC5 across various diseases. It has been implicated in the promotion of malignant diseases, suggesting a potential role in cancer biology and other pathological conditions. (PMID:25526565; 38629066)