TXNRD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TXNRD1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 11117-1-AP

Synonyms

GRIM 12, GRIM12, KDRF, thioredoxin reductase 1, Thioredoxin reductase TR1, TR, TR1, TRXR1, TXNR, TXNRD1

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Validation Data Gallery

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  WB analysis using 11117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11117-1-AP

  WB result of TXNRD1 antibody (11117-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNRD1 transfected HeLa cells.

• 

  WB analysis of MCF-7 using 11117-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11117-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11117-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNRD1 antibody (11117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of MCF-7 using 11117-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug TXNRD1 antibody (11117-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human testis tissue, human breast cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 46 publications below
• IHC: See 9 publications below
• IF: See 4 publications below

Product Information

11117-1-AP targets TXNRD1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TXNRD1 fusion protein Ag1618
• Full Name: thioredoxin reductase 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC018122
• Gene Symbol: TXNRD1
• Gene ID (NCBI): 7296
• RRID: AB_2210113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Publications

Species	Application	Title
human	WB	Redox Biol; Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.; Authors - Angel Gallego-Selles
human,mouse	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB	Cell Death Dis; Elovanoid-N34 modulates TXNRD1 key in protection against oxidative stress-related diseases; Authors - Jorgelina M Calandria
human	WB	Cancer Res; Natural allelic variations in glutathione peroxidase-1 affect it subcellular localization and function.; Authors - Soumen Bera
rat	WB	Nucleic Acids Res; Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression.; Authors - Miniard Angela C AC
human	WB	Biomed Pharmacother; TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling; Authors - Yifan Chen