TXNRD1 Monoclonal antibody, PBS Only (Capture)

Name: TXNRD1 Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 67728-1-PBS-100UG

TXNRD1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67728-1-PBS

Clone No.1B10C4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TRXR1, 1B10C4, EC:1.11.1.2, EC:1.8.1.9, Gene associated with retinoic and IFN-induced mortality 12 protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead array

WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TXNRD1 antibody (67728-1-Ig, Clone: 1B10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP51846-1
Cytometric bead array sample test of MP51846-1, TXNRD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67728-1-PBS. Detection antibody: 67728-2-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51846-1
Cytometric bead array standard curve of MP51846-1, TXNRD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67728-1-PBS. Detection antibody: 67728-2-PBS. Standard:Ag30071. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67728-1-PBS targets TXNRD1 as part of a matched antibody pair:

MP51846-1: 67728-1-PBS capture and 67728-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag30071 Product name: Recombinant human TXNRD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 199-500 aa of BC018122 Sequence: SYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCUG Predict reactive species
Full Name	thioredoxin reductase 1
Calculated Molecular Weight	55 kDa
Observed Molecular Weight	50-70 kDa
GenBank Accession Number	BC018122
Gene Symbol	TXNRD1
Gene ID (NCBI)	7296
RRID	AB_2882914
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16881
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)