Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, MJ cells, Jurkat cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, A549 cells, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67411-1-Ig targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9442 Product name: Recombinant human TYK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 901-1187 aa of BC014243 Sequence: RDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHSQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC Predict reactive species |
| Full Name | tyrosine kinase 2 |
| Calculated Molecular Weight | 1187 aa, 134 kDa |
| Observed Molecular Weight | 134 kDa |
| GenBank Accession Number | BC014243 |
| Gene Symbol | TYK2 |
| Gene ID (NCBI) | 7297 |
| RRID | AB_2882652 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P29597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| IHC protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| WB protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TYK2 Monoclonal antibody (catalog no. 67411-1-Ig) has 14 citations, all using WB or IHC. Journals include Cancer Letters, Developmental Cell, Cell Reports, Journal of Medicinal Chemistry, Antioxidants, PLoS Pathogens, Journal of Virology, International Immunopharmacology, Investigational New Drugs, Veterinary Research, Clinical and Experimental Rheumatology, and bioRxiv. Research fields span oncology (nasopharyngeal carcinoma, lymphoma, myeloproliferative neoplasms), liver fibrosis, psoriasis, atopic dermatitis, IgG4-related disease, and virology (SARS-CoV-2, African swine fever virus), focusing on JAK-STAT and interferon signaling.
| Species | Application | Title |
|---|---|---|
Cancer Lett HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma | ||
Antioxidants (Basel) Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses | ||
Chin J Nat Med Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses | ||
J Med Chem Discovery of a Highly Potent and Selective Tyrosine Kinase 2 (TYK2) Degrader with In Vivo Therapeutic Efficacy in a Murine Psoriasis Model |
