Talin-1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82856-4-RR (same clone as 82856-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82856-4-RR (same clone as 82856-4-PBS)

  IP result of anti-Talin-1 (IP:82856-4-RR, 4ug; Detection:82856-4-RR 1:3000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82856-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82856-4-RR against Human TLN1 were performed. The affinity constant is 0.215 nM.

Talin-1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 82856-4-PBS

Clone No.7D11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

7D11, ILWEQ, KIAA1027, talin 1, talin1

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Product Information

82856-4-PBS targets Talin-1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5367

  Product name: Recombinant human TLN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2193-2541 aa of BC042923

  Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH

• Full Name: talin 1
• Calculated Molecular Weight: 2541 aa, 270 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: BC042923
• Gene Symbol: Talin-1
• Gene ID (NCBI): 7094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y490
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).