Mouse Thrombopoietin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83549-7-RR (same clone as 83549-7-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

• 

  WB analysis using 83549-7-RR (same clone as 83549-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Thrombopoietin antibody (83549-7-RR, Clone: 250023B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Thrombopoietin antibody (83549-7-RR, Clone: 250023B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

Thrombopoietin Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 83549-7-PBS

Clone No.250023B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Thpo, Mpllg, TPO

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Product Information

83549-7-PBS targets Thrombopoietin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0678

  Product name: Recombinant mouse Thrombopoietin protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-356 aa of NM_001173505.1

  Sequence: SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

• Full Name: thrombopoietin
• Calculated Molecular Weight: 38KD
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001173505.1
• Gene Symbol: Thrombopoietin
• Gene ID (NCBI): 21832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40226
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).