Thrombopoietin Recombinant monoclonal antibody

• 

  WB analysis using 83549-7-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83549-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 83549-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 83549-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 83549-7-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Thrombopoietin antibody (83549-7-RR, Clone: 250023B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 83549-7-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Thrombopoietin antibody (83549-7-RR, Clone: 250023B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Thrombopoietin Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83549-7-RR

Clone No.250023B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Thpo, Mpllg, TPO

---

Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, HuH-7 cells, L02 cells, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83549-7-RR targets Thrombopoietin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0678

  Product name: Recombinant mouse Thrombopoietin protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-356 aa of NM_001173505.1

  Sequence: SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

• Full Name: thrombopoietin
• Calculated Molecular Weight: 38KD
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001173505.1
• Gene Symbol: Thrombopoietin
• Gene ID (NCBI): 21832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).