Thrombospondin 1 Polyclonal antibody, PBS Only

• 

  WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18304-1-AP (same clone as 18304-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 18304-1-AP (same clone as 18304-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 18304-1-AP (same clone as 18304-1-PBS)

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18304-1-AP (same clone as 18304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Thrombospondin 1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18304-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

THBS1, Thrombospondin-1, Glycoprotein G, THBS, THBS 1

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Product Information

18304-1-PBS targets Thrombospondin 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: thrombospondin 1
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC028145
• Gene Symbol: Thrombospondin 1
• Gene ID (NCBI): 7057
• RRID: AB_2201959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07996
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.