|Positive WB detected in||HepG2 cells, rat spleen tissue, NIH/3T3 cells, Jurkat cells, K-562 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
67241-1-Ig targets Thrombospondin 1 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
|Tested Reactivity||Human, rat, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Thrombospondin 1 fusion protein Ag29129|
|Full Name||thrombospondin 1|
|Calculated molecular weight||129 kDa|
|Observed molecular weight||160 kDa|
|GenBank accession number||NM_003246|
|Gene ID (NCBI)||7057|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.