Thrombospondin 1 Monoclonal antibody

Thrombospondin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB

Conjugate

Unconjugated

CloneNo.

2A3E1

Cat no : 67241-1-Ig

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1



Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

67241-1-Ig targets Thrombospondin 1 in WB applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Thrombospondin 1 fusion protein Ag29129
Full Name thrombospondin 1
Calculated molecular weight 129 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_003246
Gene symbol THBS1
Gene ID (NCBI) 7057
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Protocols

Product Specific Protocols
WB protocol for Thrombospondin 1 antibody 67241-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols