Tested Applications
| Positive IF/ICC detected in | bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84469-5-RR targets Tie-2 in IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | FusionProtein Predict reactive species |
| Full Name | endothelial-specific receptor tyrosine kinase |
| Calculated Molecular Weight | 126kDa |
| GenBank Accession Number | NM_001290549.1 |
| Gene Symbol | Tek |
| Gene ID (NCBI) | 21687 |
| RRID | AB_3671993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q02858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tie-2, or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 plays a significant role in several vascular diseases, including vascular malformations like venous malformations. It is also implicated in pathological vascular remodeling associated with tumor growth, where it is often overexpressed in tumor vessels.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Tie-2 antibody 84469-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
