Anti-Mouse Tie-2/CD202b Rabbit Recombinant Antibody

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  FC experiment of bEnd.3 using 98219-1-RR

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse Tie-2 Rabbit Recombinant Antibody (98219-1-RR, Clone:241753F12) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98219-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98219-1-RR against Mouse Tie-2/CD202b were performed. The affinity constant is below 1 pM.

Tie-2/CD202b {ptg:Brand} Recombinant Antibody for FC

Cat No. 98219-1-RR

Clone No.241753F12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

HYK, Endothelial tyrosine kinase, EC:2.7.10.1, CD202b, Angiopoietin-1 receptor

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98219-1-RR targets Tie-2/CD202b in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: endothelial-specific receptor tyrosine kinase
• Calculated Molecular Weight: 126kDa
• GenBank Accession Number: NM_001290549.1
• Gene Symbol: Tek
• Gene ID (NCBI): 21687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q02858
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Tie-2, also known as CD202b or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 plays a significant role in several vascular diseases, including vascular malformations like venous malformations. It is also implicated in pathological vascular remodeling associated with tumor growth, where it is often overexpressed in tumor vessels.