Tim23 Recombinant antibody

Tim23 Recombinant Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat







Cat no : 82540-1-RR


PRO1197, TIM23, TIMM23

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82540-1-RR targets Tim23 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Tim23 fusion protein Ag1620
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC062707
Gene Symbol TIMM23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)


Product Specific Protocols
WB protocol for Tim23 antibody 82540-1-RRDownload protocol
IHC protocol for Tim23 antibody 82540-1-RRDownload protocol
IF protocol for Tim23 antibody 82540-1-RRDownload protocol
IP protocol for Tim23 antibody 82540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols