Filter: AllIF/ICCFC (Intra)Affinity

IF Staining of HeLa using 83065-2-RR (same clone as 83065-2-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Tnfrsf11b antibody (83065-2-RR, Clone: 230325C3 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.
FC experiment of RAW 264.7 using 83065-2-RR (same clone as 83065-2-PBS)
1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.
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1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83065-2-RR
Biolayer interferometry (BLl) kinetic assays of 83065-2-RR against Mouse Tnfrsf11b were performed. The affinity constant is 18.1 pM.

Mouse Tnfrsf11b Recombinant monoclonal antibody, PBS Only

Name: Mouse Tnfrsf11b Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 83065-2-PBS-100UG

Tnfrsf11b Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83065-2-PBS

Clone No.230325C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230325C3, OCIF, Opg, Osteoprotegerin, PDB5

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

83065-2-PBS targets Tnfrsf11b in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0598 Product name: Recombinant mouse Tnfrsf11b protein Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 22-401 aa of BC049782.1 Sequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCRKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHANLTTEQLRALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL Predict reactive species
Full Name	tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Calculated Molecular Weight	46KD
GenBank Accession Number	BC049782.1
Gene Symbol	Tnfrsf11b
Gene ID (NCBI)	18383
RRID	AB_3670789
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6PI12
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.