Tnfrsf11b Recombinant monoclonal antibody

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  IF Staining of HeLa using 83065-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Tnfrsf11b antibody (83065-2-RR, Clone: 230325C3 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of RAW 264.7 using 83065-2-RR

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83065-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83065-2-RR against Mouse Tnfrsf11b were performed. The affinity constant is 18.1 pM.

Tnfrsf11b Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83065-2-RR

Clone No.230325C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230325C3, OCIF, Opg, Osteoprotegerin, PDB5

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83065-2-RR targets Tnfrsf11b in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0598

  Product name: Recombinant mouse Tnfrsf11b protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-401 aa of BC049782.1

  Sequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCRKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHANLTTEQLRALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL

• Full Name: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
• Calculated Molecular Weight: 46KD
• GenBank Accession Number: BC049782.1
• Gene Symbol: Tnfrsf11b
• Gene ID (NCBI): 18383
• RRID: AB_3670789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6PI12
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.