Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86992-1-RR (same clone as 86992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86992-1-RR (Tri-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86992-1-RR (same clone as 86992-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Tri-Methyl-Histone H3 (Lys27) antibody (86992-1-RR, Clone: 252044D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of / using 86992-1-RR (same clone as 86992-1-PBS)

  Dot blot analysis was used to confirm the specificity of 86992-1-RR Tri-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 86992-1-RR (same clone as 86992-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 86992-1-PBS in a different storage buffer formulation.

Tri-Methyl-Histone H3 (Lys27) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 86992-1-PBS

Clone No.252044D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

H3, H3 Me Lys27, H3 MeK27, H3 Methyl K27, H3 Methyl Lys27

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Product Information

86992-1-PBS targets Tri-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

H3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs.