Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

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• Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody (86992-1-RR)

Tri-Methyl-Histone H3 (Lys27) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Cat No. 86992-1-RR Copied!

Clone No. 252044D11

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

H3, H3 Me Lys27, H3 MeK27, H3 Methyl K27, H3 Methyl Lys27

• 

  WB analysis using 86992-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86992-1-RR (Tri-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 86992-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Tri-Methyl-Histone H3 (Lys27) antibody (86992-1-RR, Clone: 252044D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Dot Blot experiment of / using 86992-1-RR

  Dot blot analysis was used to confirm the specificity of 86992-1-RR Tri-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HeLa using 86992-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: / /
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86992-1-RR targets Tri-Methyl-Histone H3 (Lys27) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3745261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

H3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs.

Publications

What published studies show

Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody (252044D11), catalog #86992-1-RR, has 8 citations published in Nature Communications, Journal of Advanced Research, International Journal of Biological Macromolecules, Archives of Pharmacal Research, Cells, Investigative Ophthalmology & Visual Science, Journal of Inflammation Research, and Chinese Medical Journal. Associated research fields include epigenetics, cancer biology, liver fibrosis, photoreceptor gene regulation, thromboinflammation, and tissue regeneration.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Nat Commun; Mechanically heterogeneous hydrogel with cell-programmed network restructuring promotes tissue regeneration by mechano-epigenetic modulation.; Authors - Qiangjun Ling
human	WB	J Adv Res; Trained immunity-primed DLL3-targeted CAR macrophages for the eradication of small cell lung cancer.; Authors - Hangjie Ying
		Int J Biol Macromol; Nucleolar and spindle-associated protein 1 (NUSAP1) promotes thyroid cancer dedifferentiation via BCAT1-mediated metabolic-epigenetic crosstalk.; Authors - Yue Wang
human	ChIP	Arch Pharm Res; Title: Resveratrol ameliorates liver fibrosis by inhibiting ATF4 to regulate glutamine metabolism in hepatic stellate cells.; Authors - Huiya Ying
pig	WB	Cells; The Removal of H3K27me3 Promoted SLPI Transcription and Pubertal Initiation in Pigs.; Authors - Yingting He
mouse	ChIP	Invest Ophthalmol Vis Sci; Stress Responsive bZIP Transcription Factors ATF4 and BACH1 Cooperate With MAF-Family bZIP Protein NRL to Fine-Tune Rod Photoreceptor Gene Expression.; Authors - Kiam Preston Jr.