Tri-Methyl-Histone H3 (Lys27) Recombinant monoclonal antibody

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  WB analysis using 86992-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86992-1-RR (Tri-Methyl-Histone H3 (Lys27) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86992-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Tri-Methyl-Histone H3 (Lys27) antibody (86992-1-RR, Clone: 252044D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Dot Blot experiment of / using 86992-1-RR

  Dot blot analysis was used to confirm the specificity of 86992-1-RR Tri-Methyl-Histone H3 (Lys27) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row

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  ChIP experiment of HeLa using 86992-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Tri-Methyl-Histone H3 (Lys27) (86992-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Tri-Methyl-Histone H3 (Lys27) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Cat No. 86992-1-RR

Clone No.252044D11

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

H3, H3 Me Lys27, H3 MeK27, H3 Methyl K27, H3 Methyl Lys27

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells
• Positive Dot Blot detected in: / /
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• DOT BLOT: DOT BLOT : 1:10-1:100
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 3 publications below

Product Information

86992-1-RR targets Tri-Methyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3745261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

H3K27me3 is a typical transcriptional repression marker that primarily silences key developmental genes through synergistic action with polycomb group proteins. In embryonic stem cells, many developmental regulatory genes are marked by H3K27me3 and remain silent; during cell differentiation, the H3K27me3 markers for specific genes are removed, thereby activating the corresponding developmental programs.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Arch Pharm Res; Title: Resveratrol ameliorates liver fibrosis by inhibiting ATF4 to regulate glutamine metabolism in hepatic stellate cells.; Authors - Huiya Ying
mouse	IHC,WB,ChIP	Cell Signal; Role of EZH2 in regulating c-Myc stability through the E3 ubiquitin ligase HUWE1 in heart failure and its mechanisms.; Authors - Licheng Yan
human	WB	J Inflamm Res; The EZH2 Inhibitor GSK126 Alleviates Thromboinflammation in Deep Vein Thrombosis by Suppressing TLR4 Signaling via H3K27me3 Modulation.; Authors - Rudan Zhou
human	ChIP	Nat Commun; Mechanically heterogeneous hydrogel with cell-programmed network restructuring promotes tissue regeneration by mechano-epigenetic modulation.; Authors - Qiangjun Ling